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单一肌纤维培养测定法用于评估体外成人肌肉干细胞功能
JoVE Journal
Biology
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Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
Please note that all translations are automatically generated.
Click here for the English version.
单一肌纤维培养测定法用于评估体外成人肌肉干细胞功能
DOI:
10.3791/62257-v
•
09:19 min
•
February 15, 2021
•
Sören S. Hüttner
,
Christian Hayn
,
Hellen E. Ahrens
,
Manuel Schmidt
,
Henriette Henze
,
Julia von Maltzahn
1
Leibniz Institute on Aging, Fritz-Lipmann-Institute
Chapters
00:04
Introduction
00:50
Preparing Pipettes for Trituration of the Collagenase-Digested Muscles
01:54
EDL Muscle Isolation and Collagenase Digestion
03:19
Myofiber Dissociation and Culture
04:20
siRNA Transfection, Fixation, and IF Staining
06:31
Results: Culture and Transfection of Murine EDL Muscle-Derived Single Myofibres
08:34
Conclusion
Summary
Automatic Translation
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Automatic Translation
在该协议中,描述了肌肉干细胞的体外培养和功能分析方法,该方法保留了它们与其内源性生态位的大部分相互作用。
Tags
Muscle Stem Cells
Single Myofiber Culture
Ex Vivo
Niche
SiRNA Transfection
Pasteur Pipettes
Horse Serum
Ethanol
Tibialis Anterior
EDL Muscle
Digestion
Isolation Medium
Myofibers
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