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3D 생물반응기 배양에 의한 활액 중간엽 줄기세포 유래 엑소좀의 대규모 제조
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JoVE Journal
Biology
Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture
Please note that all translations are automatically generated.
Click here for the English version.
3D 생물반응기 배양에 의한 활액 중간엽 줄기세포 유래 엑소좀의 대규모 제조
DOI:
10.3791/62221-v
•
09:48 min
•
July 26, 2022
•
Li Duan
2
,
Xingfu Li
2
,
Xiao Xu
2
,
Limei Xu
2
,
Daping Wang
2
,
Kan Ouyang
2
,
Yujie Liang
3
1
Department of Orthopedics
,
The First affiliated hospital of Shenzhen University, Shenzhen Second People’s Hospital
,
2
Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology
,
Shenzhen Second People’s Hospital
,
3
Department of Child and Adolescent Psychiatry, Shenzhen Kangning Hospital, Shenzhen Mental Health Center
,
Shenzhen Key Laboratory for Psychological Healthcare & Shenzhen Institute of Mental Health
Chapters
00:05
Introduction
00:40
Human Synovial Fluid Mesenchymal Stem Cells (SF-MSCs) Culture and Identification
02:42
Microcarrier Preparation and Perfusion Bioreactor
04:18
Exosome Identification and Safety Detection
05:01
Nanoparticle Tracking Analysis (NTA) and Western Blotting
06:48
Results: Identification and Analysis of SF-MSCs and the Exosomes
08:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
여기에서는 3D 바이오리액터를 이용하여 활액 중간엽 줄기세포로부터 다수의 GMP급 엑소좀을 생산하는 프로토콜을 제시합니다.
Tags
Synovial Fluid
Mesenchymal Stem Cells
Exosomes
3D Bioreactor
GMP-grade
Cell Culture
Flow Cytometry
Microcarriers
Cell Preparation
Centrifugation
Cell Density
Cell Passage
Cell Pellet
Cell Suspension
Fluorescent Antibody
Cell Washing
Cell Detection
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