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通过冷冻软X射线断层扫描对细胞进行3D制图描述
JoVE Journal
Biology
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JoVE Journal
Biology
A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
Please note that all translations are automatically generated.
Click here for the English version.
通过冷冻软X射线断层扫描对细胞进行3D制图描述
DOI:
10.3791/62190-v
•
08:47 min
•
March 15, 2021
•
Johannes Groen
,
Andrea Sorrentino
,
Lucía Aballe
,
Robert Oliete
,
Ricardo Valcárcel
,
Chidinma Okolo
,
Ilias Kounatidis
,
Maria Harkiolaki
,
Ana Joaquina Pérez-Berná
,
Eva Pereiro
1
MISTRAL beamline
,
Alba Light Source, Cerdanyola del Valles, Spain
,
2
Beamline B24
,
Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK
Chapters
00:04
Introduction
00:51
Transmission X-Ray Microscope (TXM) Loading
01:49
TXM Brightfield Imaging
02:56
Fluorescence Mode Mosaic Acquisition
03:44
X-Ray Imaging
04:52
Tomography Acquisition
07:07
Results: Representative Cryo Soft X-Ray Tomograms
08:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,提出了一种方案,描述了冷冻软X射线断层扫描(SXT)中所需的样品制备和数据收集步骤,以以25nm半间距的分辨率对整个冷冻保存的细胞的超微结构进行成像。
Tags
3D Cartography
Cryo Soft X-ray Tomography
Cellular Imaging
Frozen Hydrated State
High-throughput
Antiviral Drugs
Gene Therapy
Transmission X-ray Microscopy
Cryo Conditions
Sample Loading
Vacuum Procedure
Brightfield Imaging
Sample Rotation
Sample Positioning
Focus Adjustment
Mosaic Acquisition
Article
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