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使用 E1A 微型工具研究 mRNA 拼接更改
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Using the E1A Minigene Tool to Study mRNA Splicing Changes
Please note that all translations are automatically generated.
Click here for the English version.
使用 E1A 微型工具研究 mRNA 拼接更改
DOI:
10.3791/62181-v
•
10:25 min
•
April 22, 2021
•
Fernanda L Basei
,
Livia A. R. Moura
,
Jörg Kobarg
1
Faculdade de Ciências Farmacêuticas
,
Universidade Estadual de Campinas
Chapters
00:05
Introduction
00:35
Cell Transfection
01:58
Cell Treatment and Collection
02:55
RNA Extraction and cDNA Synthesis
05:44
pMTE1A Minigene PCR and Gel Analysis
08:13
Results: Effects of Cisplatin and Paclitaxel on Minigene E1A Splicing Pattern
09:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议提供了一个快速和有用的工具,用于评估化疗后在替代拼接调节中具有非同寻常功能的蛋白质的作用。
Tags
E1A Minigene
MRNA Splicing
HEK 293 Cells
Cell Culture
Transfection
RNA Extraction
Alternative Splicing
Gene Expression
Chemotherapeutics
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