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Journal
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Biology
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来自肠道密码的穆林原结肠上皮单层的生成
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts
Please note that all translations are automatically generated.
Click here for the English version.
来自肠道密码的穆林原结肠上皮单层的生成
DOI:
10.3791/62156-v
•
06:57 min
•
February 06, 2021
•
Chithra K Muraleedharan
,
Jay Mierzwiak
,
Darius Feier
,
Asma Nusrat
,
Miguel Quiros
1
Department of Pathology, School of Medicine
,
University of Michigan
Chapters
00:04
Introduction
00:51
Preparation of Plates, Chamber Slides and Cell Culture Membrane Inserts
01:30
Crypt Isolation
04:09
Culturing 2D Monolayer
05:17
Results: Reliability of The Primary Epithelial Colon Monolayer Cultures
06:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在此协议中,我们描述了如何直接从肠道密码生成穆林原发性上皮结肠单层。我们提供实验方法,在透气过滤器上生成汇流单层,在划痕伤口愈合和生化研究中产生汇流单层,以及用于免疫荧光分析的稀疏和共生单层。
Tags
Primary Colon Epithelial Monolayers
Intestinal Crypts
Wound Repair
Permeability Barrier
Transepithelial Migration
Host-pathogen Interaction
Damaged Epithelial
Drug Discovery
Colon Dissection
Cell Isolation
EDTA
Cell Culture
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