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使用标准化肠道循环模型对小鼠肠道渗透性和中性粒细胞转导的功能评估
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Immunology and Infection
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JoVE Journal Immunology and Infection
Functional Assessment of Intestinal Permeability and Neutrophil Transepithelial Migration in Mice using a Standardized Intestinal Loop Model
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使用标准化肠道循环模型对小鼠肠道渗透性和中性粒细胞转导的功能评估

DOI:
10.3791/62093-v

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09:24 min

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February 11, 2021

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, ,
1Department of Pathology,University of Michigan, Ann Arbor

Chapters

  • 00:04Introduction
  • 01:29Generation of the Ileal Loop
  • 04:29Generation of the Proximal Colon Loop (pcLoop)
  • 06:37Results: JAM-A Regulates Intestinal Permeability in vivo and Promotes LTB4-dependent Recruitment of PMN into the Lumen of the pcLoop
  • 08:46Conclusion

Summary

Automatic Translation

Automatic Translation

受管制的肠道上皮屏障功能和免疫反应是炎症性肠病的标志，由于缺乏生理模型，这些特征仍然调查不力。在这里，我们描述了一个鼠标肠道循环模型，它采用了血管化和外化良好的肠道部分，以研究粘膜渗透性和白细胞在体内的招募。

Tags

Intestinal PermeabilityNeutrophil Transepithelial MigrationIntestinal Loop ModelGut Barrier FunctionInflammatory ResponseUlcerative ColitisCrohn’s DiseaseVascularized Intestinal SegmentLaparotomyCecumIleumMesenteryHBSS

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