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使用上格拉夫方法估计植物生物质的晶体纤维素含量
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Environment
Estimation of Crystalline Cellulose Content of Plant Biomass using the Updegraff Method
Please note that all translations are automatically generated.
Click here for the English version.
使用上格拉夫方法估计植物生物质的晶体纤维素含量
DOI:
10.3791/62031-v
•
12:34 min
•
May 15, 2021
•
Lavanya Dampanaboina
,
Ning Yuan
,
Venugopal Mendu
1
Department of Plant and Soil Science
,
Texas Tech University
,
2
Fiber and Biopolymer Research Institute (FBRI), Department of Plant and Soil Science
,
Texas Tech University
Chapters
00:00
Introduction
01:12
Phase I: Preparation of Samples
02:02
Phase II: Preparation of the Alcohol Insoluble Crude Cell Wall
04:52
Phase III: Removal of Hemicellulose and Lignin using Updegraff Reagent
07:10
Phase IV: Acid Hydrolysis of Cellulose into Glucose Monomers
07:49
Phase V: Anthrone Assay to Estimate the Glucose Concentration of Samples and Glucose Standard
10:04
Representative Results & Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
上格拉夫法是纤维素估计使用最广泛的方法。本次演示的主要目的是提供详细的上格拉夫协议,用于估算植物生物质样本中的纤维素含量。
Tags
Crystalline Cellulose
Updegraff Method
Plant Biomass
Cellulose Content Estimation
Cell Wall Extraction
Hemicellulose And Lignin Removal
Acid Hydrolysis
Glucose Monomers
Anthrone Assay
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