Journal
/
/
使用上格拉夫方法估计植物生物质的晶体纤维素含量
JoVE Journal
Environment
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
Estimation of Crystalline Cellulose Content of Plant Biomass using the Updegraff Method
DOI:

12:34 min

May 15, 2021

, ,

Chapters

  • 00:00Introduction
  • 01:12Phase I: Preparation of Samples
  • 02:02Phase II: Preparation of the Alcohol Insoluble Crude Cell Wall
  • 04:52Phase III: Removal of Hemicellulose and Lignin using Updegraff Reagent
  • 07:10Phase IV: Acid Hydrolysis of Cellulose into Glucose Monomers
  • 07:49Phase V: Anthrone Assay to Estimate the Glucose Concentration of Samples and Glucose Standard
  • 10:04Representative Results & Conclusion

Summary

Automatic Translation

上格拉夫法是纤维素估计使用最广泛的方法。本次演示的主要目的是提供详细的上格拉夫协议,用于估算植物生物质样本中的纤维素含量。

Related Videos

Read Article