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分离高质量鼠心房和心室肌细胞,同时测量 Ca2+ 瞬变和 L 型钙电流
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current
DOI:

06:22 min

November 03, 2020

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Chapters

  • 00:05Introduction
  • 00:51Organ Harvest
  • 01:33Enzymatic Digestion
  • 03:59Calcium Reintroduction
  • 04:57Results: Simultaneous Measurement of Calcium Currents and Transients in Atrial and Ventricular Murine Myocytes
  • 05:36Conclusion

Summary

Automatic Translation

小鼠模型允许研究心律失常发生的关键机制。为此,需要高质量的心肌细胞来进行膜片钳测量。这里描述了一种通过逆行基于酶的Langendorff灌注分离鼠心房和心室肌细胞的方法,该方法允许同时测量钙瞬变和L型钙电流。

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