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分子分析的谷类区域解剖
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Cerebellar Regional Dissection for Molecular Analysis
DOI:

08:51 min

December 05, 2020

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Chapters

  • 00:04Introduction
  • 00:54Brain Extraction and Dissection
  • 04:42RNA Extraction
  • 07:01Results: RT qPCR Relative Gene Expression in Isolated Specific Regions of the Cerebellum
  • 08:15Conclusion

Summary

Automatic Translation

不同的小脑区域被牵连到不同的行为输出中发挥作用,但潜在的分子机制仍然未知。本文描述了一种可重复和快速解剖半球、前部和后部区域以及深小脑核的方法,以便通过分离RNA和测试基因表达差异来探测分子差异。

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