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Journal
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Biology
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用于微藻模型改进的高通量代谢分析
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal
Biology
High-Throughput Metabolic Profiling for Model Refinements of Microalgae
Please note that all translations are automatically generated.
Click here for the English version.
用于微藻模型改进的高通量代谢分析
DOI:
10.3791/61913-v
•
11:07 min
•
December 04, 2021
•
Amnah Alzahmi
2
,
Sarah Daakour
,
Diana Charles El Assal
,
Bushra S. Dohai
,
Amphun Chaiboonchoe
,
Weiqi Fu
4
,
David R. Nelson
,
Alexandra Mystikou
,
Kourosh Salehi-Ashtiani
3
1
Center for Genomics and Systems Biology (CGSB)
,
New York University Abu Dhabi Research Institute
,
2
Department of Biology
,
United Arab Emirates University
,
3
Laboratory of Algal, Systems, and Synthetic Biology (LASSB), Division of Science and Math
,
New York University Abu Dhabi
,
4
Department of Marine Science, Ocean College
,
Zhejiang University
Chapters
00:07
Introduction
01:13
Phenotype Microarray Experiments
03:58
Data Extraction and Analysis
05:06
Identification of Reactions and Genes Associated with New Metabolites
06:41
Model Refinement and Evaluation
08:38
Results
10:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议展示了使用表型微阵列(PM)技术平台来定义
衣原体reinhardtii(
一种绿色微藻)的代谢需求,并完善现有的代谢网络模型。
Tags
Metabolic Profiling
Microalgae
Phenotype Micro-array
High-throughput
Chlamydomonas Reinhardtii
Metabolic Network Models
Tetrazolium-based Redox Dye
Carbon Sources
Nitrogen Sources
Phosphorus
Sulfur
Peptide Nitrogen Sources
Heterotrophic Respiration
Microplate Reader
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