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粪便（微量）RNA分离
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Immunology and Infection
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JoVE Journal Immunology and Infection
Fecal (micro) RNA Isolation
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粪便（微量）RNA分离

DOI:
10.3791/61908-v

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05:35 min

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October 28, 2020

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, ,
1Ann Romney Center for Neurologic Diseases, Department of Neurology, Partners Multiple Sclerosis Center,Brigham and Women’s Hospital and Harvard Medical School, 2Evergrande Center for Immunologic Diseases,Harvard Medical School and Brigham and Women’s Hospital

Chapters

  • 00:05Introduction
  • 00:39Feces Resuspension and Organic Extraction
  • 01:52Final RNA Isolation
  • 03:45Elution of the RNA with Nuclease-Free Water
  • 04:09Results: Electrophoresis Assay and Nanodrop Analysis of Extracted RNA
  • 05:00Conclusion

Summary

Automatic Translation

Automatic Translation

该协议从动物和人类受试者的粪便样本中分离出高质量的总RNA。商业 miRNA 分离试剂盒具有显著适应性，以优化数量和质量分离纯 RNA。RNA分离株适用于大多数下游RNA检测，如测序、微阵列和RT-PCR。

Tags

FecalMicroRNARNA IsolationDPBSAcid Phenol ChloroformEthanolFilter CartridgeWash Solution

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