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使用打开的血管窗口和活体荧光显微镜测量小鼠耳蜗中的心室血流
JoVE Journal
Medicine
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JoVE Journal
Medicine
Measurement of Strial Blood Flow in Mouse Cochlea Utilizing an Open Vessel-Window and Intravital Fluorescence Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用打开的血管窗口和活体荧光显微镜测量小鼠耳蜗中的心室血流
DOI:
10.3791/61857-v
•
09:52 min
•
September 21, 2021
•
Zhiqiang Hou
,
Yunpei Zhang
,
Lingling Neng
,
Jinhui Zhang*
,
Xiaorui Shi
1
Oregon Hearing Research Center, Department of Otolaryngology/Head & Neck Surgery
,
Oregon Health & Science University
Chapters
00:04
Introduction
02:11
Preparation of the Fluorescent-Labeled Blood Cells and Surgery to Create an Open Window
05:39
Imaging of Cochlear Blood Flow Under a Fluorescence Intravital Microscope and Video Analysis
07:54
Results: IVM Imaging of Cochlear Capillaries and Analysis of Change in Vessel Diameter and Blood Flow in the Stria
09:21
Conclusion
Summary
Automatic Translation
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Automatic Translation
使用荧光示踪剂的开放式血管窗口方法为耳蜗血流(CoBF)测量提供了足够的分辨率。该方法有助于研究正常和病理条件下小鼠CoBF的结构和功能变化。
Tags
Cochlear Blood Flow
Meniere’s Disease
Hearing Loss
Fluorescence Microscopy
Transgenic Mouse Model
Cardiac Puncture
Blood Cell Labeling
Surgical Procedure
Anesthesia
Vital Signs Monitoring
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