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使用基于多反应监测的人类脑组织蛋白质的多反应监测工作流程定量蛋白质组学工作流程
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Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
Please note that all translations are automatically generated.
Click here for the English version.
使用基于多反应监测的人类脑组织蛋白质的多反应监测工作流程定量蛋白质组学工作流程
DOI:
10.3791/61833-v
•
11:49 min
•
August 28, 2021
•
Saicharan Ghantasala*
1
,
Medha Gayathri J Pai*
2
,
Sanjeeva Srivastava
1
Centre for Research in Nanotechnology and Science
,
Indian Institute of Technology Bombay
,
2
Department of Biosciences and Bioengineering
,
Indian Institute of Technology Bombay
Chapters
00:05
Introduction
00:49
Protein Extraction from Brain Tissue
02:04
Protein Quantification and Quality Check
02:32
Protein Digestion
03:58
Desalting and Peptide Quantification
05:06
Transition List Preparation of Finalised Targets
07:30
LC Parameters
08:03
MS Parameters
08:25
Run Sequence and Instrument QC
09:08
Method Refinement
10:58
Results and Conclusions
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议旨在引入使用三倍频谱仪进行临床样本蛋白质的多反应监测 (MRM)。我们提供了系统的工作流程,从样本准备到临床样本数据分析,并采取了所有必要的预防措施。
Tags
Quantitative Proteomics
Multiple Reaction Monitoring
Targeted Proteomics
Protein Detection
Sample Preparation
Protein Extraction
Protein Digestion
Peptide Cleanup
Mass Spectrometry
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