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使用基于多反应监测的人类脑组织蛋白质的多反应监测工作流程定量蛋白质组学工作流程
JoVE Journal
Biology
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JoVE Journal Biology
Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
DOI:

11:49 min

August 28, 2021

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Chapters

  • 00:05Introduction
  • 00:49Protein Extraction from Brain Tissue
  • 02:04Protein Quantification and Quality Check
  • 02:32Protein Digestion
  • 03:58Desalting and Peptide Quantification
  • 05:06Transition List Preparation of Finalised Targets
  • 07:30LC Parameters
  • 08:03MS Parameters
  • 08:25Run Sequence and Instrument QC
  • 09:08Method Refinement
  • 10:58Results and Conclusions

Summary

Automatic Translation

该协议旨在引入使用三倍频谱仪进行临床样本蛋白质的多反应监测 (MRM)。我们提供了系统的工作流程,从样本准备到临床样本数据分析,并采取了所有必要的预防措施。

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