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肠道维利表皮的器官3D培养进行分化
JoVE Journal
Biology
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JoVE Journal Biology
3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation
DOI:

06:40 min

April 01, 2021

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Chapters

  • 00:04Introduction
  • 00:54Mice
  • 01:27Duodenum Isolation and Preparation
  • 01:54Villi Isolation by Scraping
  • 03:32Plating of Villi on BME-R1 Matrix
  • 05:02Results: Organoid Formation from the Dedifferentiating Villi
  • 05:55Conclusion

Summary

Automatic Translation

该程序描述了从小鼠肠道上皮分离,进行分化以确定其器官形成潜力。

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