JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
肠道维利表皮的器官3D培养进行分化
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation
Please note that all translations are automatically generated.
Click here for the English version.
肠道维利表皮的器官3D培养进行分化
DOI:
10.3791/61809-v
•
06:40 min
•
April 01, 2021
•
Christina Li
,
Jeel Shah
,
Kylee Wrath
,
Dahlia Matouba
,
Connor Mills
,
Kishore Punnath
,
Ansu Perekatt
1
Department of Biology and Chemical Biology
,
Stevens Institute of Technology
,
2
Department of Pharmacology
,
Pennsylvania State University College of Medicine
Chapters
00:04
Introduction
00:54
Mice
01:27
Duodenum Isolation and Preparation
01:54
Villi Isolation by Scraping
03:32
Plating of Villi on BME-R1 Matrix
05:02
Results: Organoid Formation from the Dedifferentiating Villi
05:55
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该程序描述了从小鼠肠道上皮分离,进行分化以确定其器官形成潜力。
Tags
3D Culturing
Organoids
Intestinal Villi Epithelium
De-differentiation
Stem Cell Markers
Scraping
Mesenchyme
Niche Signals
Smad4 Knockout
Beta-catenin Gain Of Function
Tamoxifen
Duodenum
PBS
Mesh Strainer
Glass Slides
Mucus
Crypts
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
一个培养人关节软骨细胞的三维系统在滑液
协议的慢病毒转导与肠组织体下游分析
小鼠视网膜色素上皮细胞的原代培养
生成用于发育和疾病建模应用的 hiPSC 衍生肠道类器官
使用活性氧敏感荧光探针分析小鼠肠道类器官中的氧化应激
从小鼠子宫建立3D子宫内膜类器官
冷冻保存的上皮隐窝仔猪肠道3D类器官的培养和细胞单层的建立
小鼠肠隐窝和单个干细胞的类器官的3D培养用于类器官研究
大鼠肠道类器官的产生和操作
具有免疫成分的基本三维 (3D) 肠道模型系统
Read Article