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使用
卡诺哈布迪炎埃莱甘斯
识别 EGFR 和 RAS 抑制剂
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Cancer Research
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JoVE Journal
Cancer Research
Identification of EGFR and RAS Inhibitors using
Caenorhabditis elegans
Please note that all translations are automatically generated.
Click here for the English version.
使用
卡诺哈布迪炎埃莱甘斯
识别 EGFR 和 RAS 抑制剂
DOI:
10.3791/61788-v
•
08:12 min
•
October 05, 2020
•
Dharini van der Hoeven
2
,
Thuy Nhu L. Truong
,
Ali Naji
,
Sabita Thapa
,
John F. Hancock
,
Ransome van der Hoeven
1
Department of Diagnostic and Biomedical Sciences, School of Dentistry
,
University of Texas Health Science Center
,
2
Department of Integrative Biology and Pharmacology, McGovern Medical School
,
University of Texas Health Science Center
Chapters
00:05
Introduction
00:26
Preparation of a Synchronous
C. elegans
Culture
02:51
Preparation of Drug Assays
04:30
Observation of the Muv Phenotype in the
let-60, let-23 and lin-1
Strains
06:38
Results: R-fendiline Alters
let-60 and let-23
Function in
C. elegans
in a Dose-dependent Manner
07:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
基因可处理的线虫
卡诺哈布迪炎的叶原体
可以用作药物发现一个简单和廉价的模型。这里描述的是一个协议,以确定抗癌治疗,抑制RAS和EGFR蛋白的下游信号。
Tags
EGFR
RAS
Caenorhabditis Elegans
Drug Discovery
Anti-EGFR
Anti-RAS
Therapeutics
E. Coli OP50
NGM Plates
Gravid Adult Worms
Worm Lysis
Embryos
L1 Larvae
M9W
Article
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