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从细胞和组织样本中分离和功能评估人类乳腺癌干细胞
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Cancer Research
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JoVE Journal Cancer Research
Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples
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从细胞和组织样本中分离和功能评估人类乳腺癌干细胞

DOI:
10.3791/61775-v

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07:03 min

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October 02, 2020

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, , , ,
1London Regional Cancer Program, 2Department of Anatomy & Cell Biology,Western University, 3Department of Oncology,Western University, 4Lawson Health Research Institute

Chapters

  • 00:05Introduction
  • 00:38Breast Cancer Cell Culture Single Cell Suspension Generation
  • 01:22Tissue Sample Single Cell Suspension Generation
  • 02:45Colony Forming Assay
  • 03:54Mammosphere Assay and 3D Culture Model
  • 05:09Results: Representative BCSC Identification and Functional Evaluation
  • 06:28Conclusion

Summary

Automatic Translation

Automatic Translation

该实验方案描述了从乳腺癌细胞和组织样品中分离BCSC以及可用于评估BCSC表型和功能的 体外 和 体内 测定。

Tags

Breast Cancer Stem CellsCell IsolationSingle Cell SuspensionALDH AssayColony Forming AssayFunctional CharacterizationTumorigenicityMetastasis

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