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Biology
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ミツバチの経口感染のためのウイルス富化接種剤の調製(
Apis mellifera
)
JoVE Journal
Biology
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JoVE Journal
Biology
Preparation of Virus-Enriched Inoculum for Oral Infection of Honey Bees (
Apis mellifera
)
Please note that all translations are automatically generated.
Click here for the English version.
ミツバチの経口感染のためのウイルス富化接種剤の調製(
Apis mellifera
)
DOI:
10.3791/61725-v
•
06:41 min
•
August 26, 2020
•
Edward M. Hsieh
,
Jimena Carrillo-Tripp
,
Adam G. Dolezal
1
Department of Entomology
,
University of Illinois
,
2
Department of Microbiology
,
Center for Scientific Research and Higher Education of Ensenada (CICESE)
Chapters
00:04
Introduction
00:41
Larval Self-Removal
02:08
Pupal Virus Injection
03:19
Viral Feeding Bioassay
04:58
Results: Representative Virus Load and Survival Curve Analyses
05:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここでは、ミツバチの蛹を除去する方法を含む大量のミツバチ非エンベロープウイルス粒子を伝播、抽出、精製、定量すること、および2番目の2つのプロトコルについて説明します。
Tags
Virus-enriched Inoculum
Oral Infection
Honey Bees
Apis Mellifera
Virus Particles
Bioassays
Virus Treatment
Bee Pupae
Virus Production
Pollinator Systems
Virus Infectivity
Mass Bee Extraction
Honey Bee Queen
Larval Self-removal
Langstroth Frame
Incubator
PBS
Virus Injection
Manual Multipipette
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