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Isolamento di fluidi prossimali per studiare il microambiente tumorale dell'adenocarcinoma pancreatico
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1Section of Pancreatic Surgery,Humanitas Clinical and Research Center-IRCCS, 2Department of Immunology and Inflammation,Humanitas Clinical and Research Center-IRCCS, 3Department of Biomedical Sciences,Humanitas University, 4Giotto Biotech S.R.L., 5Section of Bioanalytical Chemistry, Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction,Imperial College London, 6National Phenome Centre, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine,Imperial College London, 7Department of Medical Biotechnology and Translational Medicine,University of Milan
Chapters
- 00:04Introduction
- 01:03Isolation and Processing of Pancreatic Juice
- 02:20Isolation of Tumor Interstitial Fluid (TIF) from Subcutaneous Tumors
- 04:03Results: Metabolomic Content in Pancreatic Juice
- 05:14Conclusion
Il succo pancreatico è una preziosa fonte di biomarcatori per il cancro del pancreas umano. Descriviamo qui un metodo per la procedura di raccolta intraoperatoria. Per superare la sfida di adottare questa procedura in modelli murini, suggeriamo un campione alternativo, il liquido interstiziale tumorale, e descriviamo qui due protocolli per il suo isolamento.
