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اشتقاق، توسعة، المحافظة على التبريد وتوصيف خلايا الدماغ البطانية الدقيقة من الخلايا الجذعية متعددة القدرات المستحثة من الإنسان
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1Center for Genomic Medicine,Massachusetts General Hospital, 2Department of Psychiatry,Beth Israel Deaconess Medical Center, 3Chemical Biology and Therapeutic Science Program,Broad Institute of MIT and Harvard, 4Department of Psychiatry,Harvard Medical School, 5Schizophrenia and Bipolar Disorder Program,McLean Hospital
Chapters
- 00:04Introduction
- 00:38Brain Microvascular Endothelial Cell (BMEC) Differentiation from Human Induced Pluripotent Stem Cell (iPSC)
- 01:46iPSC-Derived BMEC Subculture and Purification
- 03:02Trans-Endothelial Electrical Resistance (TEER) Measurement and Analysis
- 04:09Efflux Transporter Activity and Analysis
- 05:22Results: Representative BMEC Differentiation and Characterization After Passaging, Subculture, and Cryopreservation
- 07:18Conclusion
