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利用单分子定位显微镜分析人类 iPSC 衍生肌细胞中的α-Actinin 网络
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JoVE Journal
Biology
Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
利用单分子定位显微镜分析人类 iPSC 衍生肌细胞中的α-Actinin 网络
DOI:
10.3791/61605-v
•
07:02 min
•
November 03, 2020
•
Lisa Johann
2
,
Oleksandra Chabanovska
2
,
Cajetan Immanuel Lang
,
Robert David
2
,
Heiko Lemcke
2
1
Department of Cardiac Surgery, Reference and Translation Center for Cardiac Stem Cell Therapy (RTC)
,
Rostock University Medical Center
,
2
Faculty of Interdisciplinary Research, Department Life, Light & Matter
,
University Rostock
,
3
Department of Cardiology
,
Rostock University Medical Center
Chapters
00:04
Introduction
00:27
Photoactivated Localization Microscopy (PALM) Image Acquisition Setup
01:27
PALM Image Acquisition
01:50
PALM Data Reconstruction
02:39
Reconstructed PALM Image Post Processing
03:34
Sarcomere Filament Analysis
04:41
Results: Representative Human Induced Pluripotent Stem Cell (iPSC)-Derived Cardiomyocyte alpha-Actinin Network Analysis
06:23
Conclusion
Summary
Automatic Translation
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Automatic Translation
形成适当的沙康网络对于 iPSC 衍生心肌细胞的成熟非常重要。我们提出了一种基于超分辨率的方法,允许对干细胞衍生心肌细胞的结构成熟进行定量评估,以改善促进心脏发育的培养条件。
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Single Molecule Localization Microscopy
IPSC-derived Cardiomyocytes
α-Actinin
Sarcomere Maturation
Super-resolution Imaging
PALM Imaging
TIRF Microscopy
ImageJ
Thunderstorm Plugin
Sarcomere Length Analysis
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