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小鼠肾-居民CD8+T 细胞的分离，用于流细胞测量分析
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis
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小鼠肾-居民CD8+T 细胞的分离，用于流细胞测量分析

DOI:
10.3791/61559-v

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06:07 min

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June 27, 2020

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, ,
1Department of Microbiology, Immunology and Molecular Genetics, School of Medicine,University of Texas Health Science Center at San Antonio, 2Department of Dermatology, Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital,Central South University

Chapters

  • 00:04Introduction
  • 00:56Intravascular Labeling of CD8+ T Cells
  • 02:25Enzymatic Digestion of the Kidney
  • 03:21Density Gradient Centrifugation to Enrich Lymphocytes from the Digested Kidney
  • 04:46Results: Flow Cytometry Analysis of Kidney Lymphocytes
  • 05:40Conclusion

Summary

Automatic Translation

Automatic Translation

病毒感染后，肾脏含有相对大量的CD8+T 细胞，并提供一个机会来研究非粘膜TRM 细胞。在这里，我们描述了一个方案，分离小鼠肾淋巴细胞流动细胞学分析。

Tags

Mouse KidneyCD8+ T CellsFlow CytometryBiotin Anti-CD8 AntibodyHomogenization

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