JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
通过流细胞学测定,测量自然获得的噬菌体诱导恶性疟
Plasmodium falciparum
原虫抗体
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Measuring Naturally Acquired Phagocytosis-Inducing Antibodies to
Plasmodium falciparum
Parasites by a Flow Cytometry-Based Assay
Please note that all translations are automatically generated.
Click here for the English version.
通过流细胞学测定,测量自然获得的噬菌体诱导恶性疟
Plasmodium falciparum
原虫抗体
DOI:
10.3791/61538-v
•
09:57 min
•
August 06, 2020
•
Maria del Pilar Quintana
,
Nsoh Godwin Anabire
2,3
,
Lars Hviid
4
1
Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences
,
University of Copenhagen
,
2
West African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology
,
University of Ghana
,
3
Department of Immunology
,
Noguchi Memorial Institute for Medical Research
,
4
Centre for Medical Parasitology, Department of Infectious Diseases
,
Rigshospitalet
Chapters
00:04
Introduction
00:33
THP-1 and Infected Erythrocyte (IE) Culture Setup
01:58
Parasite Staining and Opsonization Plate Preparation
03:47
THP-1 Cell and Phagocytosis Plate Preparation
04:35
Phagocytosis Assay
06:21
Flow Cytometry Acquisition and Analysis
07:34
Results: Representative Evaluation of Parasite-IE Opsonization and Phagocytosis
09:21
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议的总体目标是指导如何测量自然接触恶性疟原虫感染的血清或血浆中的抗体的能力,以对抗和诱导寄生虫感染的红细胞(IEs)的噬菌体。
Plasmodium falciparum
Tags
Phagocytosis
Plasmodium Falciparum
Flow Cytometry
Antibodies
Parasites
Erythrocytes
THP-1 Cells
Parasitemia
Ethidium Bromide
Opsonization
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
选择
恶性疟原虫寄生虫对人脑血管内皮细胞
一个<em>在体外</em>共感染模型研究<em>恶性疟原虫</em人原发性单核细胞源性的免疫细胞-HIV-1相互作用
一个简单的协议介导的血小板聚集<em>恶性疟原虫</em>受感染的红细胞在一个资源匮乏的设置
分离<em>恶性疟原虫</em>晚期感染的红细胞由磁性装置
高产净化<em>恶性疟原虫</em>裂殖子适用于Opsonizing抗体检测
在
啮齿动物
疟原虫
原虫的
体内
评估和裂殖子入侵流式细胞仪
方法调查小RNA和核糖体占用的调控作用<em>恶性疟原虫</em
流式细胞仪为基础的检测方法的NK细胞功能的监控
评估视网膜小胶质细胞吞噬功能<em>在体内</em>使用基于流式细胞术流量测定
A Flow Cytometry-Based Cytotoxicity Assay for the Assessment of Human NK Cell Activity
Read Article