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Journal
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Immunology and Infection
/
蝙蝠主要组织兼容性复杂类 I 的稳定性和结构与异质β
2
- 微球蛋白
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β
2
-Microglobulin
Please note that all translations are automatically generated.
Click here for the English version.
蝙蝠主要组织兼容性复杂类 I 的稳定性和结构与异质β
2
- 微球蛋白
DOI:
10.3791/61462-v
•
11:17 min
•
March 10, 2021
•
Di Zhang*
1,2
,
Kefang Liu*
3,4
,
Dan Lu*
2,5
,
Pengyan Wang
2
,
Qingxu Zhang
2
,
Peipei Liu
,
Yingze Zhao
,
Yan Chai
,
Jianxin Lyu
,
Jianxun Qi
,
William J. Liu
2
1
School of Laboratory Medicine and Life Sciences
,
Wenzhou Medical University
,
2
NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention
,
Chinese Center for Disease Control and Prevention
,
3
Faculty of Health Sciences
,
University of Macau
,
4
CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology
,
Chinese Academy of Sciences
,
5
Savaid Medical School
,
University of Chinese Academy of Sciences
Chapters
00:04
Introduction
00:54
E. coli
Transformation
01:46
Culture Inoculation
02:59
Bacteria Harvest
03:28
Inclusion Body Purification
04:27
MHC Complex Refolding
04:53
MHC H Chain and Beta
2
m Injection and Dilution
05:42
Refolded Protein Concentration Determination
06:51
Crystallization, Data Collection, and Processing
07:41
Structure Determination and Analyses
08:33
Results: Representative Bat MHC I Beta2-Microglobulin Stability and Structure Analysis
10:21
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了通过从不同物种获得2-微球蛋白(β 2米)替代的潜在β获得稳定的主要组织相容
性复合物(MHC)I类的实验方法
。调查了由同源和异质
β 2
米稳定下来的MHC I的结构比较。
Tags
Bat MHC Class I
Heterologous β2-microglobulin
Hybrid MHC Class I
E. Coli Transformation
Recombinant Protein Expression
IPTG Induction
Bacterial Cell Pellet
PBS
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