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Biology
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MCF7細胞単層における細胞基質接着面積と細胞形状分布の定量化
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Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers
Please note that all translations are automatically generated.
Click here for the English version.
MCF7細胞単層における細胞基質接着面積と細胞形状分布の定量化
DOI:
10.3791/61461-v
•
06:46 min
•
June 24, 2020
•
Maciej Wakula
,
Anna Balcerak
,
Urszula Smietanka
,
Mateusz Chmielarczyk
,
Ryszard Konopiński
,
Ewa A. Grzybowska
1
Maria Sklodowska-Curie National Research Institute of Oncology
Chapters
00:04
Introduction
00:33
Confluent Monolayer Generation
01:06
Focal Adhesion Analysis
03:13
Manual Cell Shape Analysis
04:04
Automated Cell Shape Analysis
04:54
Results: Representative Focal Adhesion and Cell Shape Distribution Analyses
06:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
本稿では、1)焦点接着のサイズと数および2)細胞形状指標およびMCF7細胞のコンラエント単層の共焦点画像からの分布の定量化について説明する。
Tags
Cell-substrate Adhesion
Cell Shape
MCF7 Cell Monolayers
Quantification
Confocal Microscopy
ImageJ
Focal Adhesion Analysis
Background Subtraction
Binary Conversion
Particle Analysis
ImageJ Macro
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