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精确的大脑映射，在小鼠中执行神经-免疫动力学的重复体内振动成像
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Immunology and Infection
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JoVE Journal Immunology and Infection
Precise Brain Mapping to Perform Repetitive In Vivo Imaging of Neuro-Immune Dynamics in Mice
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精确的大脑映射，在小鼠中执行神经-免疫动力学的重复体内振动成像

DOI:
10.3791/61454-v

•

08:17 min

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August 07, 2020

•

, ,
1Center for Brain Immunology and Glia (BIG),University of Virginia, 2Department of Neuroscience,University of Virginia

Chapters

  • 00:04Introduction
  • 00:51Mouse Preparation for Cranial Window Implantation
  • 02:20Cranial Window Implantation Surgery
  • 03:57Two-photon Brain Mapping and Imaging
  • 05:24Results: Visualizing Microglial Dynamics In Vivo
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了一种慢性颅窗植入技术，可用于神经-胶质-血管结构、相互作用和功能在健康和患病条件下的纵向成像。它是颅内成像方法的补充替代品，虽然通常倾向于，但具有一些关键限制。

Tags

In Vivo ImagingTwo-photon MicroscopyNeuro-immune DynamicsBrain MappingRepetitive VisualizationCellular DynamicsBrain PlasticityNeurodegenerationCX3CR1 GFP MouseCranial Window ImplantationStereotactic SurgeryBupivacaineDexamethasoneBetadineHydrogen PeroxideDental DrillCyanoacrylate Glue

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