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Modélisation de l’infection par le virus de l’hépatite B dans les cellules non hépatiques 293T-NE-3NRs
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1Stem Cell Research Center,Shantou University Medical College, 2The Center for Reproductive Medicine,Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College, 4Medical Research Center, Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University, 5Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute,General Autority – City of Scientific Researches and Technological Applications
Chapters
- 00:04Introduction
- 01:36HepG2.2.15 Cell Culture
- 03:04Detection of HBV DNA in Supernatant Concentrate
- 04:23In Vitro Infection of HepG2-NE and 293T-NE-3NRs Cells
- 05:27HepG2.2.15 Co-culture with HepG2-NE / 293T-NE-3NRs / 293T-NE
- 06:41Results: HBcAg Expression in 293T-NE, 293T-NE-3NRs and HepG2-NE
- 07:47Conclusion
Ce manuscrit décrit un protocole détaillé pour l’infection par le virus de l’hépatite B (VHB) dans de nouvelles cellules 293T (293T-NE-3NR, exprimant des NTCP humains, HNF4α, RXRα et PPARα) et des cellules hépatiques traditionnelles (PleG2-NE, exprimant le NTCP humain).
