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测量小鼠分离和孵育成熟骨骼肌中胰岛素和收缩刺激的葡萄糖摄取
JoVE Journal
Biology
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JoVE Journal
Biology
Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice
Please note that all translations are automatically generated.
Click here for the English version.
测量小鼠分离和孵育成熟骨骼肌中胰岛素和收缩刺激的葡萄糖摄取
DOI:
10.3791/61398-v
•
08:01 min
•
May 16, 2021
•
Rasmus Kjøbsted
,
Kohei Kido
,
Jeppe K. Larsen
,
Nicolas O. Jørgensen
,
Jesper B. Birk
,
Ylva Hellsten
,
Jørgen F. P. Wojtaszewski
1
Section of Molecular Physiology, Department of Nutrition, Exercise and Sports
,
University of Copenhagen
,
2
Section of Integrative Physiology, Department of Nutrition, Exercise and Sports
,
University of Copenhagen
Chapters
00:04
Introduction
00:39
Mouse Soleus and Extensor Digitorum Longus (EDL) Harvest
03:15
Insulin-Stimulated Glucose Uptake
04:18
Contraction-Stimulated Glucose Uptake
04:57
Skeletal Muscle Homogenization and Radiolabeled 2-Deoxyglucose and Mannitol Determination
05:58
Results: Representative Mouse Soleus and EDL Muscle Metabolic Activity Analysis
07:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
肌肉葡萄糖摄取的完全调节对于维持全身葡萄糖稳态非常重要。该方案在描述各种生理干预对全身葡萄糖代谢的影响时,评估孤立和孵育成熟骨骼肌中胰岛素和收缩刺激的葡萄糖摄取。
Tags
Glucose Uptake
Skeletal Muscle
Insulin
Contraction
Ex Vivo Model
Mouse
Soleus
Tibialis Anterior
EDL
Incubation
Dissection
Suture Loops
Forced Transducers
Basal Incubation Medium
Oxygen
Carbon Dioxide
Article
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