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Visualizing Synaptic Degeneration in Adult Drosophila in Association with Neurodegeneration
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Visualizing Synaptic Degeneration in Adult Drosophila in Association with Neurodegeneration

Visualizing Synaptic Degeneration in Adult Drosophila in Association with Neurodegeneration

DOI:
10.3791/61363-v

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06:10 min

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May 13, 2020

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,
1Department of Biological Sciences,Lehigh University

Chapters

  • 00:04Introduction
  • 00:41Thorax Isolation and Fixation
  • 02:12Flash Freezing and Thorax Bisection
  • 03:41Structural Staining
  • 04:08Tissue Mounting
  • 04:57Results: Representative Synaptic Integrity Assessment
  • 05:46Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this procedure is to dissect the dorsal longitudinal muscle (DLM) tissue to assess the structural integrity of DLM neuromuscular junctions (NMJs) in neurodegenerative disease models using Drosophila melanogaster.

Tags

Synaptic DegenerationAdult DrosophilaNeurodegenerationNeuromuscular JunctionsALSParkinson’sHuntington’sAlzheimer’s DiseaseDissectionAnesthesiaForcepsDissection ScissorsFormaldehydeCryoprotective GlovesLiquid NitrogenFeather BladePasteur Pipette

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