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Karakterisering van immuuncellen en pro-inflammatoire mediatoren in de pulmonale omgeving
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment
DOI:

09:00 min

June 24, 2020

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Chapters

  • 00:04Introduction
  • 00:45Lung Tissue Harvest
  • 02:50Lung Tissue Homogenization
  • 03:44Lung Tissue Dissociation and Single Cell Isolation
  • 05:21Flow Cytometric Lung Immune Cell Niche Analysis
  • 06:27Results: Representative Immune Cell Population and Chemokine Expression Characterization
  • 08:35Conclusion

Summary

Automatic Translation

Dit protocol beschrijft het gebruik van flowcytometrie om de veranderingen in de samenstelling van immuuncellen, het cytokineprofiel en het chemokineprofiel in de pulmonale omgeving te identificeren na voorbijgaande occlusie van de middelste hersenslagader, een muizenmodel van ischemische beroerte.

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