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使用双边子宫内电渗透的管道,以询问遗传对啮齿动物行为的影响
JoVE Journal
Biology
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Biology
A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior
Please note that all translations are automatically generated.
Click here for the English version.
使用双边子宫内电渗透的管道,以询问遗传对啮齿动物行为的影响
DOI:
10.3791/61350-v
•
06:59 min
•
May 21, 2020
•
Ashley L. Comer
2,3,4
,
Balaji Sriram
,
William W. Yen
,
Alberto Cruz-Martín
2,3,4,6
1
The Graduate Program for Neuroscience
,
Boston University
,
2
Department of Biology
,
Boston University
,
3
Neurophotonics Center
,
Boston University
,
4
Center for Systems Neuroscience
,
Boston University
,
5
Research and Early Development
,
Biogen
,
6
Department Pharmacology and Experimental Therapeutics
,
Boston University
Chapters
00:04
Introduction
01:08
Preparation for Surgery
02:24
In Utero Electroporation Surgery
04:51
Results: Social Behavior in Juvenile and Adult Mice
06:34
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
最近发现的疾病相关基因在神经精神病发病机制中的作用仍然模糊不清。子宫电化技术的改性双边技术允许在大量神经元中进行基因转移,并检查基因表达变化对社会行为的因果关系。
Tags
Bilateral In Utero Electroporation
Genetic Influences
Rodent Behavior
Gene Expression
Neurological Diseases
Genetic Animal Lines
Neurological Disorders
Gene Transfer
Pathology
Novel Therapies
Embryo Manipulation
Gene Transfer
Pregnant Dam
Surgery
Sterile
DNA Solution
Glass Pipette
Anesthesia
Analgesia
Abdominal Incision
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