Journal
/
Neuroscience
/
A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents

A High-Throughput Image-Guided Stereotactic Neuronavigation and Focused Ultrasound System for Blood-Brain Barrier Opening in Rodents

DOI:
10.3791/61269-v

•

08:02 min

•

July 16, 2020

•

, , , , , , , ,
1Amsterdam UMC, Vrije Universiteit Amsterdam, Pediatric Oncology,Cancer Center Amsterdam, 2Princess Máxima Center for Pediatric Oncology, 3Imaging Division,Utrecht University

Summary

Automatic Translation

Automatic Translation

The blood-brain barrier (BBB) can be temporarily disrupted with microbubble-mediated focused ultrasound (FUS). Here, we describe a step-by-step protocol for high-throughput BBB opening in vivo using a modular FUS system accessible for non-ultrasound experts.

Tags

High-throughputImage-guidedStereotacticNeuronavigationFocused UltrasoundBlood-brain BarrierGliomaPrecision MedicineDrug EvaluationSonoporationTransducerStereotactic PlatformAnimal Preparation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke

A Benchtop Approach to the Location Specific Blood Brain Barrier Opening using Focused Ultrasound in a Rat Model

Targeted Neuronal Injury for the Non-Invasive Disconnection of Brain Circuitry

Focused Ultrasound Induced Blood-Brain Barrier Opening for Targeting Brain Structures and Evaluating Chemogenetic Neuromodulation

Author Spotlight: Understanding Microtubule Network in Drosophila Neuromuscular Junctions

Author Spotlight: A Personalized Approach Towards Investigating Alzheimer's Disease Using an In Vitro Blood-Brain Barrier Model

Author Spotlight: Applications of TEER Detection to Assess Cell Barrier Integrity

Read Article