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使用自动成像系统测量 iPSC 的汇合度。

JoVE Journal
Biology

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JoVE Journal Biology

Measuring the Confluence of iPSCs Using an Automated Imaging System

Please note that all translations are automatically generated. Click here for the English version.

使用自动成像系统测量 iPSC 的汇合度。

DOI:

10.3791/61225-v

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11:39 min

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June 10, 2020

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Valentina Magliocca², Maria Vinci, Tiziana Persichini, Franco Locatelli, Marco Tartaglia, Claudia Compagnucci

¹Genetics and Rare Diseases Research Division,Ospedale Pediatrico Bambino Gesù, IRCCS, ²Department of Science,University Roma Tre, ³Department of Onco-hematology, Gene and Cell Therapy,Ospedale Pediatrico Bambino Gesù, IRCCS

Chapters

00:04Introduction
00:4296-Well Plate Coating
02:49Induced Pluripotent Stem Cell (iPSC) Culture Maintenance
05:03Cell Confluence Characterization
06:53Cytoskeletal Microfilament Characterization
09:02Results: Representative Analyses of Cell Confluence on Differently Coated Vessels
10:57Conclusion

Summary

Automatic Translation

该方案的目标是比较不同的细胞外基质（ECM）包被条件，以评估差异涂层如何影响诱导多能干细胞（iPSC）的生长速率。特别是，我们的目标是创造条件以获得iPSC培养物的最佳生长。

Tags

IPSCs Induced Pluripotent Stem Cells Automated Imaging System Confluence Extracellular Matrix Basement Membrane Matrix Laminin Vitronectin Fibronectin Mouse Embryonic Fibroblasts Cell Culture Cryopreservation

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