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Journal
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Immunology and Infection
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Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
DOI:
10.3791/61178-v
•
05:59 min
•
May 14, 2020
•
Yujia Lin
2
,
Jingna Xue
,
Shan Liao
1
Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University
,
Harbin Medical University
,
2
Inflammation Research Network, Department of Microbiology, Immunology and Infectious Diseases, Snyder Institute for Chronic Diseases, Cumming School of Medicine
,
University of Calgary
Chapters
00:04
Introduction
00:40
Preparation of Materials
01:11
Preparation of the Animal for Surgery
01:47
Surgical Suturing of Afferent Lymphatic Vessels
03:47
Tracking the Lymph Flow
04:31
Results: Selective Prevention of Lymph Flow
05:09
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
A protocol to block lymph flow by surgical suturing of afferent lymphatic vessels is presented.
Tags
Lymph Flow
Lymphatic Vessels
Afferent Lymphatic Vessels
Popliteal Lymph Nodes
Intradermal Injection
Evans Blue Dye
Suture
Mouse
Anesthesia
Microsurgery
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