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Neuroscience
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成人ラット尿膀胱からの一次ニューロンとグリアの分離と培養
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder
Please note that all translations are automatically generated.
Click here for the English version.
成人ラット尿膀胱からの一次ニューロンとグリアの分離と培養
DOI:
10.3791/61177-v
•
06:18 min
•
May 23, 2020
•
Rui Wang
,
Zi-tong Huang
,
Wen-kang Ren
,
Jiao Zhang
,
Yao Zhang
,
Bo Tan
,
Ping Huang
2
,
Hong-ying Cao
2
1
School of Pharmaceutical Sciences
,
Guangzhou University of Chinese Medicine
,
2
Dongguan & Guangzhou University of Chinese Medicine Cooperative Academy of Mathematical Engineering for Chinese Medicine
,
Guangzhou University of Chinese Medicine
,
3
School of Basic Medical Sciences
,
Guangzhou University of Chinese Medicine
Chapters
00:04
Introduction
00:43
Bladder Harvest
02:11
Two-step Bladder Digestion
04:14
Results: Phase-contrast and Immunofluorescence Images of Primary Cells Isolated from the Rat Bladder
05:36
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、一次ニューロンとラット膀胱からのグリア分離のための反復可能なプロトコルを確立し、さらなる細胞実験を試みる。
Tags
Primary Neurons
Adult Rat
Urinary Bladder
Cell Culture
Co-culture
Isolation
Digestion
Krebs Solution
Centrifugation
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