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人体心室心肌细胞与颤音-切切心肌切片分离
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Biology
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JoVE Journal
Biology
Isolation of Human Ventricular Cardiomyocytes from Vibratome-Cut Myocardial Slices
Please note that all translations are automatically generated.
Click here for the English version.
人体心室心肌细胞与颤音-切切心肌切片分离
DOI:
10.3791/61167-v
•
11:19 min
•
May 10, 2020
•
Dominik J. Fiegle
,
Tilmann Volk
2
,
Thomas Seidel
2
1
Institute of Cellular and Molecular Physiology
,
Friedrich-Alexander University Erlangen-Nürnberg
,
2
Muscle Research Center Erlangen (MURCE)
,
Friedrich-Alexander-Universität Erlangen-Nürnberg
Chapters
00:04
Introduction
00:41
Preparing the Cardiac Tissue
02:27
Using the Vibratome
04:00
Tissue Digestion and Dissociation
07:03
Removing Mechanical Uncoupling Agent
08:08
Results: Vibratome-Cut Slices Yield Viable Cardiomyocytes
10:33
Conclusion
Summary
Automatic Translation
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Automatic Translation
呈现是将人类和动物心室心肌细胞与振动切切心肌切片分离的协议。耐钙细胞(高达200个细胞/毫克)的高产量可以从少量组织(<50毫克)中获得。该协议适用于暴露于冷缺血的心肌,长达36小时。
Tags
Cardiomyocyte Isolation
Vibratome
Myocardial Slices
Agarose Embedding
Electrophysiology
Cardiac Disease
Human Heart
Animal Heart
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