JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
肺上皮細胞における
シュードモナス
感染に及ぼすタバコの煙の影響の研究
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Studying Effects of Cigarette Smoke on
Pseudomonas
Infection in Lung Epithelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
肺上皮細胞における
シュードモナス
感染に及ぼすタバコの煙の影響の研究
DOI:
10.3791/61163-v
•
09:15 min
•
May 11, 2020
•
Tiao Li
,
Chen Long
,
Kristen V. Fanning
,
Chunbin Zou
1
Acute Lung Injury Center of Excellence, Pulmonary, Allergy, Critical Care Medicine, Department of Medicine
,
University of Pittsburgh School of Medicine
Chapters
00:04
Introduction
00:42
Cigarette Smoking Extract (CSE) Preparation
01:39
Pseudomonas
Culture
02:16
Cell Culture and CSE Treatment
03:33
Bacterial Infection and Gentamycin Treatment
04:24
Determination of Bacterial Concentration Using the Drop Plate Method
04:55
RT-qPCR Detection of Bacterial 16S rRNA
07:00
Results: CSE-Treatment Increases Bacterial Load
08:23
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
ここで説明する、タバコの煙抽出物が肺上皮細胞における細菌のコロニー形成にどのように影響するかを研究するプロトコルである。
Tags
Cigarette Smoke
Pseudomonas
Lung Epithelial Cells
Bacterial Load
Cell Culture
Smoke Extraction
Bacterial Infection
Cell Counting
Cell Suspension
Cell Preparation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
共培養モデルの
緑膿菌
バイオフィルム
M細胞による細菌の取り込みを評価するパイエル板腸管ループアッセイを連結したマウスのアプリケーション
に対する抗生物質の有効性をテストするための人工痰培地の使用<em>緑膿菌(Pseudomonas aeruginosa)</em>肺嚢胞性線維症へのより適切な条件で
機能と分子生物学的研究のためのプライマリマウスII型肺胞上皮細胞のフローサイトメトリ分離
長期的な慢性<em>緑膿菌</emマウスにおける>気道感染
緑膿菌
誘発肺傷害モデル
フローから白血球の補充に間質細胞の影響を分析する
PM10における粒子結合のBet V 1アレルゲンのフローサイトメトリー分析
チェンカバーガラスモデルを用いたバイオフィルム開発に関する痰の効果を可視化
細胞毒性の評価と肺癌精密カット スライスで物質の免疫調節効果
Read Article