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Immunology and Infection
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使用实时细胞分析监测宿主外的流感病毒存活率
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell Analysis
Please note that all translations are automatically generated.
Click here for the English version.
使用实时细胞分析监测宿主外的流感病毒存活率
DOI:
10.3791/61133-v
•
09:02 min
•
February 20, 2021
•
Thomas Labadie
,
Quentin Grassin
,
Christophe Batéjat
,
Jean-Claude Manuguerra
,
India Leclercq
3
1
Department of Infection Biology
,
London School of Hygiene and Tropical Medicine
,
2
Institut Pasteur, Unité Environnement et Risques Infectieux, Cellule d’Intervention Biologique d’Urgence (CIBU)
,
3
Cellule Pasteur
,
Université de Paris
Chapters
00:04
Introduction
00:41
Influenza A Virus Stock Preparation
02:38
Cell Infection Quantity Determination
04:59
CIT
50
Value and Multiplicity of Infection (MOI) Correlation
06:01
Influenza A Virus (IAV) Kinetics
07:10
Results: Representative Influenza Virus External Host Survival Monitoring
08:20
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里报道的是使用实时监测受感染细胞的电阻抗来定量感染性病毒颗粒的方案。通过量化甲型流感病毒在模拟环境条件下的不同物理化学参数下的衰变,提出了该方法的实际应用。
Tags
Influenza Virus
Real-time Cell Analysis
Viral Quantification
Cytopathic Effect
MDCK Cells
Virus Propagation
Hemagglutinin
Viral Storage
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