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成人マウスおよびラット心筋細胞の分離、トランスフェクション、長期培養
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Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes
Please note that all translations are automatically generated.
Click here for the English version.
成人マウスおよびラット心筋細胞の分離、トランスフェクション、長期培養
DOI:
10.3791/61073-v
•
09:17 min
•
October 10, 2020
•
Perwez Alam
,
Bryan D. Maliken
,
Malina J. Ivey
,
Shannon M. Jones
,
Onur Kanisicak
1
Department of Pathology and Laboratory Medicine, College of Medicine
,
University of Cincinnati
Chapters
00:00
Introduction
01:14
Preparation & Materials
02:49
Heart Extraction
04:15
Heart Digestion & Single-Cell Suspension
05:59
Cardiomyocyte Plating & Transfection
08:13
Results
08:50
Conclusion
Summary
Automatic Translation
English (Original)
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Nederlands (Dutch)
français (French)
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עברית (Hebrew)
italiano (Italian)
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español (Spanish)
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Automatic Translation
ここでは、成体マウスおよびラット心筋細胞の分離、トランスフェクション、および長期培養のためのプロトコルを提示する。
Tags
Cardiomyocytes
Adult Mouse And Rat
Isolation
Transfection
Long-term Culture
Proliferative Potential
Gene Expression
Cytokine Expression
Injury Response
Optimized Method
Survival Rate
Transfection Efficiency
Surgical Instruments
Profusion Apparatus
Heparin
Myocyte Buffer
Enzyme Solution
Anesthesia
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