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Journal
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Immunology and Infection
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实时研究全血-内皮相互作用和血栓动力学的体外微流体疾病模型
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time
Please note that all translations are automatically generated.
Click here for the English version.
实时研究全血-内皮相互作用和血栓动力学的体外微流体疾病模型
DOI:
10.3791/61068-v
•
09:19 min
•
May 24, 2020
•
Xue D. Manz
,
Hugo J. Albers
3
,
Petr Symersky
,
Jurjan Aman
,
Andries D. van der Meer
,
Harm Jan Bogaard
,
Robert Szulcek
5,6
1
Department of Pulmonary Diseases, Amsterdam UMC
,
VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS)
,
2
BIOS Lab-on-a-Chip group
,
University of Twente
,
3
Applied Stem Cell Technologies Group
,
University of Twente
,
4
Department of Cardio-thoracic Surgery, Amsterdam UMC
,
VU University Medical Center
,
5
Institute of Physiology
,
Charité-Universitätsmedizin
,
6
German Heart Center
Chapters
00:04
Introduction
00:59
Primary Human Pulmonary Arterial Endothelial Cell (PAEC) Isolation and Culture
02:43
Flow Chambers and PAEC Monolayer Preparation
04:00
Washed Human Whole Blood Preparation
04:50
Flow System Assembly
05:41
Washed Whole Blood Perfusion
06:44
Results: Representative Thrombus Formation Characterization
08:29
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一个体外血管疾病模型,以研究整个血液与患者衍生的内皮的相互作用。该系统允许研究各种情况下原发内皮细胞的血栓特性。该方法特别适用于凝血不同阶段的原位血栓形成性和抗凝治疗。
Tags
In Vitro
Microfluidic
Disease Model
Whole Blood
Endothelial
Blood Clot
Thrombogenicity
Anticoagulant
Inflammatory
Personalized Medicine
Pulmonary Artery
Fibroronectin
Endothelial Cell
CD144
Flow Cytometry
Flow Chamber
Gelatin
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