Journal
/
Immunology and Infection
/
使用主机范围和视觉选择快速、无缝地生成重组痘病毒
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
Please note that all translations are automatically generated. Click here for the English version.

使用主机范围和视觉选择快速、无缝地生成重组痘病毒

DOI:
10.3791/61049-v

•

09:25 min

•

May 24, 2020

•

, , , ,
1Department of Medial Microbiology and Immunology,School of Medicine, University of California Davis, 2Department of Microbiology and Immunology,University of Texas Medical Branch at Galveston

Chapters

  • 00:04Introduction
  • 01:03Recombination Vector Generation
  • 03:47Recombinant Virus Generation
  • 06:31Results: Representative Recombinant Poxvirus Generation
  • 08:36Conclusion

Summary

Automatic Translation

Automatic Translation

这是一种使用宿主范围选择和重组病毒的可视化识别来生成"无疤痕"重组病毒的方法。

Tags

Recombinant PoxvirusesHost Range SelectionFluorescent MarkersHomologous RecombinationTransient Dominant SelectionVaccinia VirusForeign Gene ExpressionVaccinesPCRDNA Gel ExtractionDNA AssemblyE. Coli Transformation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

质粒DNA重组流感病毒的一代

RNAi筛选参与宿主因素牛痘病毒感染使用果蝇细胞

胶粘剂基于磁带的采样和荧光组合原位杂交快速检测沙门氏菌新鲜农产品

在FDA批准的Vero细胞疫苗开发产生重组沙粒

可扩展的高通量选择从噬菌体展示合成抗体库

从排序的永生B细胞产生的重组人IgG单克隆抗体

分析<em>小肠结肠炎耶尔森菌</em>效应易位到宿主细胞中使用β-内酰胺酶效应融合体

链霉菌发育突变体的视觉和显微评价

细菌病原体在细胞和有机水平宿主反应探针中的应用

重组呼吸道合胞病毒的生成、扩增和滴定

Read Article