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Journal
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Immunology and Infection
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多能干细胞人类巨噬细胞的产生与表征
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Production and Characterization of Human Macrophages from Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
多能干细胞人类巨噬细胞的产生与表征
DOI:
10.3791/61038-v
•
08:05 min
•
April 16, 2020
•
Martha Lopez-Yrigoyen
2
,
Alisha May
,
Telma Ventura
,
Helen Taylor
,
Antonella Fidanza
,
Luca Cassetta
,
Jeffrey W. Pollard
,
Lesley M. Forrester
1
Centre for Regenerative Medicine, Scottish Centre for Regenerative Medicine
,
University of Edinburgh
,
2
Centre for Reproductive Health, The Queen’s Medical Research Institute
,
University of Edinburgh
Chapters
00:04
Introduction
00:51
Passaging Human iPSCs
01:46
Human iPSC Differentiation to Macrophages
04:33
High-Throughput Phagocytosis Assay
05:07
Results: Phenotype and Function of iPSC-Derived Macrophages
07:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了人类诱导多能干细胞的巨噬细胞的强健生成,以及其后续表征的方法。细胞表面标记表达、基因表达和功能测定用于评估这些iPSC衍生巨噬细胞的表型和功能。
Tags
Macrophage
Pluripotent Stem Cells
Cell Therapy
Genetic Manipulation
Cell Culture
Cell Differentiation
Embryoid Bodies
Cell Passaging
Cell Harvesting
Media Replenishment
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