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卡诺哈布迪蒂斯精英热
休克反应的测量诱导标准化方法
JoVE Journal
Environment
This content is Free Access.
JoVE Journal
Environment
Standardized Methods for Measuring Induction of the Heat Shock Response in
Caenorhabditis elegans
Please note that all translations are automatically generated.
Click here for the English version.
卡诺哈布迪蒂斯精英热
休克反应的测量诱导标准化方法
DOI:
10.3791/61030-v
•
06:01 min
•
July 03, 2020
•
Nicole L. Golden
,
Rosemary N. Plagens
,
Karen S. Kim Guisbert
,
Eric Guisbert
1
Department of Biomedical and Chemical Engineering and Sciences
,
Florida Institute of Technology
Chapters
00:04
Introduction
00:54
Fluorescent Imaging of HSR Reporters
02:58
Thermorecovery Assay
03:32
Results: HSR Induction Analysis
05:17
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,提出了标准化的协议,以评估在卡诺哈布迪蒂斯埃
利根
的热休克反应(HSR)的诱导使用RT-qPCR在分子水平,荧光报告器在细胞水平,并在有机体水平的光覆盖。
Tags
Heat Shock Response
Caenorhabditis Elegans
Thermal Tolerance
Thermal Recovery
Synchronized Worm Population
Developmental Timing
Heat Shock
GFP Synthesis
Agarose Pad
Levamisole
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