JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
反向转录循环介导的等温放大(RT-LAMP)测定,用于对罗非鱼湖病毒进行特定和快速检测
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus
Please note that all translations are automatically generated.
Click here for the English version.
反向转录循环介导的等温放大(RT-LAMP)测定,用于对罗非鱼湖病毒进行特定和快速检测
DOI:
10.3791/61025-v
•
07:47 min
•
May 18, 2020
•
Theerawut Phusantisampan
,
Pattarasuda Rawiwan
3
,
Sri Rajiv Kumar Roy
,
Malinee Sriariyanun
,
Win Surachetpong
3
1
Department of Biotechnology, Faculty of Applied Science
,
King Mongkut’s University of Technology North Bangkok (KMUTNB)
,
2
Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine
,
Kasetsart University
,
3
Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies
,
Kasetsart University
,
4
Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS)
,
King Mongkut’s University of Technology North Bangkok (KMUTNB)
Chapters
00:03
Introduction
00:59
RNA Extraction
02:50
Primer Design
05:20
Demonstration of RT-Lamp Assay
07:10
Results
08:19
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
与传统RT-PCR技术相比,我们提出了一种RT-LAMP测定方法,用于在相对较短的时间内使用简单的仪器检测罗非鱼中的TiLV。该议定书可能有助于控制TiLVD的流行传播,特别是在发展中国家。
Tags
RT-LAMP
Reverse Transcription Loop-mediated Isothermal Amplification
Tilapia Lake Virus
TiLV
Rapid Detection
RNA Extraction
Primer Design
PrimerExplorer
LAMP Primers
Segment 3
Aquaculture
Tilapia
Virus Detection
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
胶粘剂基于磁带的采样和荧光组合
原位
杂交快速检测
沙门氏菌
新鲜农产品
从现场收集到的蚊子传染病毒Vero细胞培养法检测
微波辅助植物病毒病的快速诊断用透射电子显微镜
流感病毒的高通量检测方法
HIV逆转录酶和整合酶抑制剂的快速筛选
环介导等温扩增(LAMP)法进行的物种特异性检测<em>艾美耳球虫</em>可感染鸡
鼻湿巾从猪流感病毒检测和隔离
冻干环介导等温扩增试剂的检测中的发展<em>贝氏柯克斯体</em
在体内
应用免疫模型检测抗原特异性 T 细胞细胞功能的试验
Zika 病毒特定诊断表位发现
Read Article