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使用纳米-牛顿分辨率力转换器从骨骼肌肉活检中隔离肌异物并确定收缩函数
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JoVE Journal Biology
Isolating Myofibrils from Skeletal Muscle Biopsies and Determining Contractile Function with a Nano-Newton Resolution Force Transducer
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使用纳米-牛顿分辨率力转换器从骨骼肌肉活检中隔离肌异物并确定收缩函数

DOI:
10.3791/61002-v

•

07:55 min

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May 07, 2020

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, , , ,
1Department of Physiology,Amsterdam UMC, 2Department of Kinesiology and Physical Education, Faculty of Education,McGill University, 3IONOptix BV

Chapters

  • 00:04Introduction
  • 00:50Myofibril and Mounting Needle Preparation
  • 02:08Experiment Initialization
  • 04:03Tension Redevelopment Rate Measurement
  • 05:04Stepwise Stretch
  • 05:33Results: Representative Active and Passive Tension Analyses
  • 07:03Conclusion

Summary

Automatic Translation

Automatic Translation

此处介绍的是一个协议，用于评估具有纳米-牛顿分辨率的条纹肌肉肌异半孔的收缩特性。该协议采用基于干涉测量的光学力探头的设置。此设置生成具有高信噪比的数据，并可评估 myofibril 的收缩动力学。

Tags

MyofibrilsSkeletal Muscle BiopsiesContractile FunctionNano-Newton Resolution Force TransducerSarcomere DysfunctionSarcomeric ProteinsPermeabilized CardiomyocytesHomogenizationMyofibril SuspensionRelaxing SolutionPoly-HEMA Coated SlideShellac Ethanol GlueMounting NeedleMicro-manipulatorsSarcomere LengthVessel FunctionVideo ImagingMicroscope Stage

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