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Journal
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Immunology and Infection
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Opsono-坚持性检测,以评估疫苗开发中针对
无烟杆菌
和其他封装病原体的功能抗体
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Opsono-Adherence Assay to Evaluate Functional Antibodies in Vaccine Development Against
Bacillus anthracis
and Other Encapsulated Pathogens
Please note that all translations are automatically generated.
Click here for the English version.
Opsono-坚持性检测,以评估疫苗开发中针对
无烟杆菌
和其他封装病原体的功能抗体
DOI:
10.3791/60873-v
•
13:47 min
•
May 19, 2020
•
Jennifer Chua*
1
,
Donald J. Chabot*
1
,
Tammy Putmon-Taylor
3
,
Arthur M. Friedlander
1
Bacteriology Division
,
United States Army Medical Research Institute of Infectious Diseases
,
2
Headquarters Division
,
United States Army Medical Research Institute of Infectious Diseases
,
3
Bioqual
Chapters
00:04
Introduction
01:21
Plating of the Cell Line, RAW 264.7 and Bacterial Culture
06:29
FITC-labeling of Bacteria and Bacterial Opsonization
08:46
Adherence Assay and Fluorescent Labeling of Mammalian Cells
10:22
Microscopy
11:33
Results: Representative Micrographs Collected During an OAA Experiment to Evaluate the Efficacy of an Anthrax Vaccine
13:01
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
opsono-依从性测定是分析抗体在疫苗开发中的opsonic功能的替代方法。
Tags
Opsono-adherence Assay
Functional Antibodies
Vaccine Development
Bacillus Anthracis
Encapsulated Pathogens
RAW 264.7 Cells
Bacillus Anthracis Capsule
Biosafety Level 2
India Ink Staining
Bacterial Suspension
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