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Viabilidad mejorada para cultivos de hidrogel 3D Ex vivo de xenoinjertos derivados del paciente en una plataforma microfluídica perfundida
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1Department of BioSciences,Rice University, 2Mimetas US Inc, 3Department of Genitourinary Medical Oncology Research, Division of Cancer Medicine,The University of Texas MD Anderson Cancer Center, 4Mimetas B.V., 5Department of Bioengineering,Rice University, 6Department of Diagnostic and Biomedical Sciences,The University of Texas Health Science Center, 7Sheikh Ahmed Center for Pancreatic Cancer Research,The University of Texas MD Anderson Cancer Center
Chapters
- 00:04Introduction
- 01:09PDX (Patient-Derived Xenografts) Dissociation and Initial Purification of Stromal Component
- 03:17Density Gradient Centrifugation-Based Separation of PDX-Derived Clusters from Single Cells
- 06:03Hydrogel Preparation and Microfluidic Plate Seeding
- 10:12Results: Cell Staining and Viability Assessment
- 11:16Conclusion
Este protocolo demuestra métodos para permitir el cultivo in vitro extendido de xenoinjertos derivados del paciente (PDX). Un paso mejora la viabilidad general de los cultivos de racimos multicelulares en hidrogeles 3D, a través de la eliminación directa de células individuales no viables. Un paso secundario demuestra las mejores prácticas para el cultivo de PDX en una plataforma microfluídica perfundida.
