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Journal
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Immunology and Infection
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使用免疫荧光显微镜鉴定帕芬-嵌入式猫动脉血栓中中性粒细胞外陷阱
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用免疫荧光显微镜鉴定帕芬-嵌入式猫动脉血栓中中性粒细胞外陷阱
DOI:
10.3791/60834-v
•
08:48 min
•
March 29, 2020
•
Laetitia Duler
,
Nghi Nguyen
,
Eric Ontiveros
,
Ronald H. L. Li
1
Veterinary Medical Teaching Hospital, School of Veterinary Medicine
,
University of California, Davis
,
2
Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine
,
University of California, Davis
,
3
Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine
,
University of California, Davis
Chapters
00:04
Introduction
01:15
Deparaffinization, Rehydration, and Heat-Induced Antigen Retrieval
02:29
Immunolabeling and Autofluorescence Quenching
05:13
Neutrophil Extracellular Trap Identification
06:08
Results: Phase Contrast and Immunofluorescence Images
07:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种使用热诱导抗原检索和双免疫标记协议识别甲醛固定和石蜡嵌入的猫科动物心性动脉血栓中嗜中性粒细胞外陷阱(NETs)的方法。
Tags
Neutrophil Extracellular Traps
Feline Arterial Thrombi
Immunofluorescence Microscopy
Antigen Retrieval
Citrullinated Histone H3
Thrombosis
Veterinary Species
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