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人工撕裂制剂对暴露于干燥后人类角膜上皮细胞代谢活动的影响
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal
Medicine
Effect of Artificial Tear Formulations on the Metabolic Activity of Human Corneal Epithelial Cells after Exposure to Desiccation
Please note that all translations are automatically generated.
Click here for the English version.
人工撕裂制剂对暴露于干燥后人类角膜上皮细胞代谢活动的影响
DOI:
10.3791/60812-v
•
06:29 min
•
May 02, 2020
•
Rekha Rangarajan
,
Howard A. Ketelson
,
Richard Do
,
David J. McCanna
,
Adeline Suko
,
Daryl Enstone
,
Lakshman N. Subbaraman
,
Jaya Dantam
,
Lyndon W. Jones
1
Alcon Vision, LLC
,
2
Centre for Ocular Research and Education (CORE), School of Optometry & Vision Science
,
University of Waterloo
Chapters
00:05
Introduction
00:52
Cell Preparation
02:26
No Desiccation Protocol
03:41
Desiccation Protocol
04:54
Results: Effect of Dry Eye Lipid Enhanced Products on Cell Viability and Desiccation Protection
05:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议的目的是评估不同的人工撕裂配方是否能使用体外模型保护人类角膜上皮细胞免受干燥。在接触人工撕裂配方和干燥后,对人体角膜上皮细胞进行代谢活性评估。
Tags
Corneal Epithelial Cells
Desiccation
Artificial Tear Formulations
Metabolic Activity
Cell Culture
Cell Viability Assay
Fluorescence Measurement
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