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定量微模式细胞细胞外细胞异细胞的时态参数
JoVE Journal
Biology
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JoVE Journal Biology
Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells
DOI:

10:21 min

September 16, 2020

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Chapters

  • 00:04Introduction
  • 01:11Micropattern Preparation
  • 03:20Cell Seeding
  • 04:37Exocytosis Imaging
  • 05:16Exocytosis Coordinate Acquisition
  • 06:56Single Cell Spatial Analysis
  • 07:34Results: Representative Lysosomal Exocytosis and Spatial Analyses
  • 09:44Conclusion

Summary

Automatic Translation

在微模型细胞上对细胞外细胞的活成像允许这个过程的空间定量。使用微模式进行形态学规范化是揭示细胞过程空间分布的一般规则的杰出工具。

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