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Cancer Research
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结合条件敲击和适应球形成测定研究患者衍生胃癌干细胞的干细胞相关基因
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Cancer Research
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JoVE Journal
Cancer Research
Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
结合条件敲击和适应球形成测定研究患者衍生胃癌干细胞的干细胞相关基因
DOI:
10.3791/60799-v
•
09:24 min
•
May 09, 2020
•
Jixian Xiong*
1
,
Yuting Li*
1
,
Xiangyu Tan*
1
,
Li Fu
1
Guangdong Key Laboratory of Genome Stability and Human Disease Prevention, Department of Pharmacology and Shenzhen University International Cancer Center
,
Shenzhen University School of Medicine
Chapters
00:04
Introduction
01:02
Generation of Inducible Knockdown GCSCs Lines
03:31
Generation of Stable Transfected Cell Lines
06:12
Sphere Formation Assay
06:54
Results: Inducible Knockdown of Clusterin Inhibits GCSC Self-renewal Capacity
08:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在该协议中,我们提出了一个实验设计,使用条件敲制系统和适应球形分析,以研究聚类对患者衍生GCSC的茎的影响。该协议可以很容易地适应研究在不同类型的CSC的茎相关基因的体外和体内功能。
Tags
Cancer Stem Cells
Conditional Knockdown
Sphere Formation Assay
Stemness-associated Genes
Gastric Cancer
Lentivirus
293T Cells
Transfection
Virus Concentration
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